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Image Search Results
Journal: Journal of applied microbiology
Article Title: Microelectronic-sensing assay to detect presence of Verotoxins in human faecal samples.
doi: 10.1111/j.1365-2672.2012.05321.x
Figure Lengend Snippet: Figure 3 Dynamic monitoring of cytotoxic response to Verotoxins. Vero cells were seeded into the 16· sensor device, and cell prolifera- tion was then monitored. Once the Vero cells and verotoxin-produc- ing Escherichia coli supernatants (at different dilutions as indicated) were added into testing well, the cell killing or proliferation was then dynamically monitored. (a) The dynamic cytotoxic response to differ- ent dilutions of Verotoxins; (b) Dose response between cell index and different dilutions of Verotoxins. (c) Correlation between Cell Index values from the real-time cell electronic sensing assay at 15 h and absorbance from the crystal violet assay at 48 h. ( ) Control; ( ) Dilution 1:20; ( ) Dil 1:80; ( ) 1:320; ( ) 1:640; ( ) 1:1280; ( ) 1:2560.
Article Snippet: Materials, bacterial strains and
Techniques: Crystal Violet Assay, Control
Journal: Journal of applied microbiology
Article Title: Microelectronic-sensing assay to detect presence of Verotoxins in human faecal samples.
doi: 10.1111/j.1365-2672.2012.05321.x
Figure Lengend Snippet: Figure 4 Real-time cell electronic sensing (RT-CES) Verotoxin assay was used to determine four bacterial strains: E. coli O157:H7 strain ATCC 35150, non-E. coli O157:H7 strain ATCC 25955, as well as clinical identified E. coli O157:H7 strains Edm and Cal. Cytotoxicity data indicated that Cal is a non-E. coli O157:H7 that does not produce any Verotoxins although O157 is positive in both PCR assay and immunoassay. (a) Crystal vio- let assay (b) RT-CES assay. (c) PCR assay: E. coli-Cal is negative for both Verotoxin I and II although it is positive for O157 gene. 1. Verotoxin I gene standard, 2. Verotoxin II gene standard, 3. Verotoxin genes for ATCC 25955, 4. Verotoxin gene for E. coli-Edm, 5. Verotoxin gene for E. coli ATCC 35150, 6. Verotoxin gene for E. coli-Cal, 7. O157 gene for ATCC 25955, 8. O157 gene for E. coli-Edm, 9. O157 gene for E. coli ATCC 35150, 10. O157 gene for E. coli-Cal, 11. Control, and 12. Molecular Marker. ( ) without Ab; ( ) with Ab.
Article Snippet: Materials, bacterial strains and
Techniques: Control, Marker